Enhanced Recovery of L-Glutaminase by the Optimization of A Three-Phase Partitioning System Using the Taguchi Doe Methodology

Quaisar Perween; Vishal Anand; Hare Ram Singh; Santosh Kumar Jha

Quaisar Perween Department of Bio-Engineering, BIT Mesra, Ranchi, Jharkhand, India
Vishal Anand Department of Bio-Engineering, BIT Mesra, Ranchi, Jharkhand, India
Hare Ram Singh Department of Bio-Engineering, BIT Mesra, Ranchi, Jharkhand, India
Santosh Kumar Jha Department of Bio-Engineering, BIT Mesra, Ranchi, Jharkhand, India

Keywords: L-Glutaminase, Taguchi DOE, Acute Lymphoblastic Leukemia (ALL), Three Phase Partitioning, Pseudomonas fluorescens.

Abstract

L-Glutaminase is an anti-leukemic enzyme produced by number of microbes. The three-phase partitioning method of Iso-octane followed by the ammonium sulphate precipitation was used to purify the L-glutaminase produced by Pseudomonas fluorescens. The selected process parameters viz. concentration of ammonium sulphate, Iso-octane to broth ratio, temperature and pH were optimized by Taguchi orthogonal array design of experiment (DOE). Overall, 28.19%, enhanced recovery of L-glutaminase was achieved after the optimization of selected factors.