In Vitro Xanthine Oxidase Inhibitor Activity of Ethanol Extract and Fraction Roselle Calyx (Hibiscus sabdariffa L.)

Sri Wahyuningsih1; Elin Yulinah Sukandar; Sukrasno

Sri Wahyuningsih1 School of Pharmacy Bandung Institute of Technology jl. Ganeca no 10 Bandung, Indonesia.
Elin Yulinah Sukandar School of Pharmacy Bandung Institute of Technology jl. Ganeca no 10 Bandung, Indonesia.
Sukrasno School of Pharmacy Bandung Institute of Technology jl. Ganeca no 10 Bandung, Indonesia.

Keywords: Hibiscus sabdariffa L, xanthine oxidase, extracts, fractions, antihyperuricemia.

Abstract

Objective: The objective of this study is to evaluate xanthine oxidase inhibitor activity of ethanol extract and fraction of roselle calyx (Hibiscus sabdariffa L) in vitro. Methods: The principle of measurement of xanthine oxidase activity inhibition is measuring the reduce of uric acid amount formed from the reaction catalyzed by xanthine oxidase, The uric acid is formed from the reaction between hypoxanthine and xanthine with xanthine oxidase enzyme. In this study allopurinol is use as reference drug. Result: The results showed that ethanol extract (100 μg/ml) can inhibit the activity of xanthine oxidase enzyme 25.13 %, water fraction inhibit 25.81% and the ethyl acetate fraction inhibit 32.25 %. with IC50 value of extract is 1294.37, IC50 water fraction 830.71 and IC50 value of ethyl acetat fraction is 290.62 μg/ml . Conclusion: Ethanol extract and fractions Roselle calyx have xanthine oxidase inhibitor activity with IC50 value of extract is 1294.37, IC50 water fraction 830.71 and IC50 value of ethyl acetat fraction is 290.62 μg/ml.