Tea tissue culture: In vitro shoot regeneration and callus induction in tea

Abstract

Camellia sinensis, or Tea, is one of the most important crop plants in the world. Biotechnological interventions in the propagation of tea have become necessary as conventional methods prove insufficient. From the present study, MS medium supplemented with 0.5 mg L"' Kinetin was found to be effective in inducing in vitro shooting from apical and nodal explants derived from 3-month-old seedlings of Camellia sinensis cv. UPASI-9. For callus induction in in vitro grown leaves, MS medium supplemented with 0.5 mg L"' NAA and 0.5 mg L"' 2,4-D was found to be most effective.