Genetic homology between the CMS (A) and their alloplasmic maintainer (B) line in chilli pepper through SSR markers

Abstract

The purpose of this study was to assess the genetic similarity between the CMS (A) and the maintainer (B) lines using 120 SSR markers. Almost all the amplified markers showed monomorphic bands between the CMS lines and corresponding B lines. Between CMS463D2A and CMS463D2B (alloplasmic maintainer line) and between CMS463L9A and CMS463L9B, polymorphic banding patterns were observed by a marker (Hpms 2-13) for chromosome 1. After eight cycles of backcrossing (BC8F1) and selection, the genome recovery of the recurrent parent in all the CMS lines was estimated to be 100%, except CMS463D2A and CMS463D2B, and between CMS463L9A and CMS463L9B where the recovery was 99.01%, and 98.9%, respectively. Similarly, for the fruit weight, fruit length, fruit width, and pericarp thickness, the paired t-test was nonsignificant for mean value of all the CMS A- and CMS Blines except CMS4624A and CMS4624B for fruit length, and CMS4626A and CMS4626B for fruit width, where the differences were significant.