Callus induction and plant regeneration of tomato through anther culture

Sandeep  Kumar; SK   Jindal; NK    Sarao; MS   Dhaliwal

doi:10.61180/vegsci.2020.v47.i1.04

Sandeep Kumar Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004
SK Jindal Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004
NK Sarao School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004
MS Dhaliwal Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004

DOI: https://doi.org/10.61180/vegsci.2020.v47.i1.04

Keywords: Growth regulators, organogenesis, Solanum lycopersicum L.

Abstract

Anthers of 15 genotypes were cultured on MS medium having different concentration of auxins and cytokinins to induce callusing. Of the different media compositions used, callus induction was observed in eight genotypes on MS medium supplemented with 2ip (1mg/l) and IAA (2mg/l). The percent callus formation was maximum in genotype Wild Texas (88.00) and minimum in TLBNRH-1214(4%). The calli obtained from the anthers of the eight genotypes were cultured on different regeneration media. However, the regeneration was observed only in calli of Wild Texas with percent regeneration of 0.66 on MS media supplemented with zeatin (0.5mg/l). After rooting on MS medium containing IBA (0.1mg/l), plantlets were hardened and transferred to pots.