Electrophoretic protein profile and ISSR marker based genetic relationship study in ridge gourd [Luffa acatangula (Roxb.)L.]

Abstract

An experiment was conducted to study the biochemical as well as molecular traits among eight ridge gourd inbred lines by using SDS-PAGE analysis and ISSR marker data. The entire seed protein profile comprised of 24 protein bands, distributed into four major zones A, B, C and D in the increasing order of electrophoretic mobility as analysed by SDS-PAGE. Maximum sixteen bands were found in PRG 120 and minimum thirteen bands were observed in PCPGR 7256 and PRG 142. Similarity index value ranged from 25% to 100%. Hundred percent similarities were observed for genotype PCPGR 7256 with PRG 117. Genotype PRG 131 showed least similarity (25%) with PRG 132 as PRG 137 with PRG 7. The UPGMA clustering divided all the genotypes into two major clusters (I and II) with 43% similarity among them. Cluster I comprised of 5 genotypes and cluster II comprised of 3 genotypes. Among 20 ISSR markers, only 4 were selected due to their polymorphic bands. A total of 27 bands were produced and the number of loci ranged from 6 to 7. The per cent polymorphism varied from 66.67 to 85.71 with an average of 74.07. The similarity matrix value ranged from 43% to 90%. The maximum similarity value was recorded for PCPGR 7256 (0.90) with PRG 142. The lowest similarity was recorded for PCPGR 7256 (0.43) with PRG 137. In ISSR profile based dendogram eight genotypes were classified into two main clusters. Cluster I comprised of 5 genotypes with 62% similarity among themselves whereas cluster II comprised of 3 genotypes with 67% similarity. The information generated in the present study could be further utilized for ridge gourd improvement programme in future.