Callus mediated regeneration through cotyledonary and hypocotyl explants in chilli (Capsicum annuum L.)

JK Ranjan; AK Chakrabarti; SK Singh; Pragya .

doi:10.61180/vegsci.2018.v45.i1.14

JK Ranjan Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi
AK Chakrabarti Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi
SK Singh Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi
Pragya . Germplasm Exchange Division, National Bureau of Plant Geteic Resources, New Delhi

DOI: https://doi.org/10.61180/vegsci.2018.v45.i1.14

Keywords: Indirect organogenesis, in vitro regeneration, Capsicum annuum L., Cotyledonary explant, hypocotyl explant

Abstract

Chilli is recalcitrant to in vitro regeneration and many factors such as genotypes, explants and growth regulators play important role in in vitro indirect organogenesis. The present study was undertaken to standardize callus mediated regeneration protocol through indirect organogenesis in four Indian chilli cultivars namely, KtPL-19, Pusa Sadabahar, ArCH-001 and Salem. Cotyledonary and hypocotyls explants were excised from 21-day old in vitro raised seedlings and subjected to different treatments for callusing and subsequent regeneration. Full-strength Murashige and Skoog (MS) medium was found better than half strength MS medium (MS/2) and B5 medium for culture initiation. Callus induction in both cotyledonary leaf (98.11%) and hypocotyl (97.97%) explants was the maximum on MS medium containing 5.0 mgL-1 2, 4 dicholorophenoxy acetic acid (2, 4-D) +1.0 mgL-1 kinetin (Kn). For callus multiplication, the combination consisting of MS+ 3.0 mgL-1 2,4-D+0.5 mgL- 1 kinetin was found to be the best. Shoot bud induction was achieved on hypocotyl derived callus, when thidiazuron (TDZ) (1.5 mgL-1) was used as a source of cytokinin in MS medium.