An Observation on Morphogenetic Response of Marchantia polymorpha subsp. ruderalis Bischl. and Boissel.-Dub. in Different Culture Media

Abstract

The growth pattern of Marchantia polymorpha subsp. ruderalis, using gemmae as explant, in different culture media in combination with auxin and cytokinins has been studied. The plant growth was maximum in ½ KNOP's followed by KNOP's, Hoagland and Murashige and Skoog media. The normal growth of gemmae was inhibited at higher concentrations of hormone (1 mg L-1 Kinetin + 1 mg L-1 Auxin, 1.5 mg L-1 Kinetin + 1.5 mg L-1 Auxin, 0.5 mg L-1 2,4D) and callus like tissue was produced, while at lower concentrations (0.1 mg L-1 Kinetin+0.1 mg L-1 Auxin) gemmae developed normally and rhizoids were profusely produced on the surface of young thalli. Cultured thalli were transferred to the soil in pots and introduced in moss house.