Comet Assay: A Strong Tool for Evaluating DNA Damage and Comprehensive Guidelines for Plant Cells
Keywords:
Heavy metals, DNA damage, Single cell gel electrophoresis, Tail length, Tail moment
Abstract
Heavy metals affect plant system in various toxic ways including morphophysiological alterations and genotoxic damages inside a plant cell. The extent of DNA damage under any genotoxic agents can be effectively measured in single cells applying comet assay approach. Comet assay primarily measures DNA strand breakage in single cells and its use has increased in different areas: clinical applications, human monitoring, radiation biology and genetic ecotoxicology. This paper is a review of the detailed protocol and precautions to be taken while performing comet assay and may have been slightly modified from other original protocols according to the plant, organ, cell type, etc. In conclusion, the study reviewed in this paper demonstrate that the comet assay application in plants provides a reliable, sensitive and rapid system for the study of environmental genotoxicity caused by heavy metals.
Published
2017-07-31
How to Cite
1.
Agnihotri A, Seth C. Comet Assay: A Strong Tool for Evaluating DNA Damage and Comprehensive Guidelines for Plant Cells. IJPE [Internet]. 31Jul.2017 [cited 3Jul.2024];3(02):65-2. Available from: https://myresearchjournals.com/index.php/IJPE/article/view/1223
Section
Review Article
Copyright (c) 2017 International Journal of Plant and Environment
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