A new strain of Papaya ring spot virus infecting ivy gourd (Coccinia grandis L.) in India

Nagendran  K; Manoranjitham  SK; Karthikeyan   G

doi:10.61180/vegsci.2022.v49.i1.08

Nagendran K Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641003, Tamil Nad
Manoranjitham SK ICAR-Indian Institute of Vegetable Research, Varanasi– 221305, Uttar Pradesh
Karthikeyan G ICAR-Indian Institute of Vegetable Research, Varanasi– 221305, Uttar Pradesh

DOI: https://doi.org/10.61180/vegsci.2022.v49.i1.08

Keywords: Cucurbits virus; Potyvirus; RT-PCR; Vegetable virus; Papaya ring spot virus

Abstract

Ivy gourd (Coccinia grandis L.) plants showing virus like symptoms such as mosaic mottling and thickening of leaf lamina were collected from Tindivanam, Tamil Nadu. Through Reverse transcription polymerase chain reaction, infection of potyvirus was confirmed with the yield of desired amplicon (~1.0 kb) using universal primer pair. Sequence analysis of amplified product showed the infection of Papaya ring spot virus (PRSV). Further association of PRSV was confirmed with PRSV specific primer pair (GK PRSV F/R). The nucleotide sequence analysis showed identity of coat protein region shared only 84% with PRSV isolate from Thailand in BLAST analysis. In phylogenetic analysis, PRSV infecting ivy gourd formed a separate cluster from other PRSV isolates. This confirms the association of distinct strain of PRSV on Ivy gourd in India.